At1g79830(GC5) ORF Clone

产品内容

pLinker1-At1g79830------------------------2 μg

TE buffer--------------------------------------500 μl

产品信息

产品名称：At1g79830(GC5) ORF Clone

产品货号：QA1g79830

别称：GC5;At1g79830;F19K16.21

产品类型：ORF克隆载体

基因ID：At1g79830

转录本：AT1G79830.4

载体：pLinker1

抗性：kanR

ORF 长度：2931 bp

片段物种：拟南芥（Arabidopsis thaliana）

NCBI gene ID：844322

基因摘要

This gene is predicted to encode a protein that functions as a Golgi apparatus structural component known as a golgin in mammals and yeast. A fluorescently-tagged version of GC5 co-localizes with Golgi markers, and this localization appears to be replicated using the C-terminal (139 aa) portion of the protein. The C-terminal portion of the protein can also specifically interact with two members of the Rab family of GTPases (RabH1b and RabH1c).

ORF 核苷酸序列

>At1g79830(GC5) ORF Clone

ACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCCAGTGAATTCGAGCTCGGTACCTCGCGAATGCATCTAGATATC

ATGGCTTGGTTCAGTGGCAAAGTTTCTCTGGGAGGATTCCCAGATCTCACTGGCGCTGTCAATAAATTCCAGGAGAGCGTTAAAAACATTGAAAAGAATTTCGACAACGCCCTTGGCTTCGACGACAAGTCCGATTCTGCCGCTGAAGATGCAGCTTCAAGTATGTGGCCACCTGCAGTTGATACCAAAAGCCTCTTTGATCCCGTTATGTCCTTCATGGGTAACACCTCTGATGAGAAACCTGATACATTGGAAGACTCTGTGCGTACAGAAAATCCGTCTCAAATTGAACAAAAAGAAGAAGAAGCTGGATCGGTTAAGCTAGCTACTGAACAAGCAGTATCTGTTGAAGCAAATAAAGAAACAAACGTGAGAAGAGAAGCTGATCAAGCAGATAATCCTGAGGTAACAGAAACTGTTGTTTTGGATCCCAAAGATGATGAACCGCAATCGCAGATACTTCTCGAAGAGTCCTCTGAATATTCTCTTCAGACTCCTGAATCCTCAGGTTACAAGACTAGTCTTCAACCTAATGAAAAGCTGGAAATGACAGCTTCTCAAGATTCACAGCCCGAGCAACCCAAGTCAGAGGCTGAGGAATCACAGCCTGAGGATTCTGAAGCAAAAGAGGTTACTGTAGAAAACAAAGACACTGTTCACTCCCCTGTGTTAGATGGACAGCATAAGATTACTTATATGGATGAGACAACAAATGAACAAGAAATTCTGGGTGAAAATCTGGAAGGGAGAACCTCGTCTAAAAATTTTGAAGTTTCACCAGATATCAATCATGTAAATAGGATAGAGTCCCCTGTTGCTCATCCGTCTTTAATTTTTGAGTCTGATGGTTCTCCTTACGAGTCTTCTATACCAAAGAGATCGTCGTCAGATGAAATTTCGGAGAGAATTGTGGATTTTGTTTCTCGTGAAATAGATTCAAGACTGGATACTAGTGAGTTAAATGAAAGCCAGCGTTCAAGCTCTGCGACAAATGTTTCCGACTCTGCTGATGTTATTCTGGAATTAGAGAAGACCAAAAAGGAAATAAAAATGTTGGAAAATGCCCTGCAAGGTGCTGCAAGGCAAGCTCAGGCAAAGGCTGATGAGATTGCGAAGTTGATGCATGAAAATGAGCAGCTAAAATCTGTCACTGAGGATTTGAAGAGGAAATCTAACGAAGCTGAAGTCGAGTCTTTGCGAGAAGAATACCATCAGAGAGTTGCAACTCTTGAGAGGAAGGTTTATGCTCTCACCAAAGAGCGGGATACACTTAGAAGAGAACAGAACAAGAAAAGTGATGCTGCGGCCCTTTTGAAAGAAAAGGATGAAATAATAAATCAAGTTATGGCTGAAGGCGAAGAGCTTTCAAAGAAACAGGCTGCTCAAGAAGCTCAGATCAGGAAGCTAAGGGCTCAGATTAGAGAGGCAGAAGAAGAAAAGAAAGGACTGATCACAAAGCTTCAGTCGGAAGAAAATAAGGTCGAAAGCATTAAAAGGGACAAGACAGCTACAGAAAAGCTGCTGCAAGAGACCATAGAAAAACATCAAGCTGAACTTACTTCTCAGAAAGACTATTACTCAAACGCATTAGCTGCAGCAAAGGAAGCTCAAGCGTTAGCTGAGGAGCGTACCAACAATGAAGCGAGGTCAGAGTTAGAGAATCGTCTAAAGGAGGCTGGAGAGCGGGAATCTATGCTAGTCCAGGCGCTGGAAGAATTGAGGCAAACCTTAAGCAAAAAGGAGCAACAGGCAGTGTATAGGGAAGACATGTTCCGTGGAGAAATTGAGGACCTTCAAAGACGCTATCAGGTCAAATCAAGATGTTTCGTTGTCTCTATGCATATTTCTTTCTTTGGTTATCGCCTTTTGGCTAGTGAGCGAAGGTGTGAGGAATTAATCACACAGGTTCCTGAGTCTACAAGGCCTCTCCTAAGACAGATTGAGGCTATGCAGGAAACTTCATATAGAACAGCAGAAGCTTGGGCTGCTGTAGAAAGAACTCTGAATTCTCGATTGCAGGAAGCAGAAAGTAAAGCTGCAACAGCTGAAGAACGAGAGCGCTCTGTGAATGAACGCCTCTCTCAAACATTATCAAGGATCAATGTTCTAGAGGCCCAGCTTTCATGTCTCAGAGCAGAACAGGGCCAGTTAAGCAAGTCACTTGAGAAAGAGAGGCAGAGAGCAGCAGAAAATAGACAGGAGTATCTGGCAGCTAAGGAGGAAGCAGATACCCTAGAAGGTCGAGCAAACCAACTTGAAGTTGAGATAAGGGAACTTCGGAGAAAACATAAGCAAGAGTTGCAGGAAGTGCTACTCCACAACGAGCTTATTCAAAAGGACCTAGAGAGGGAGAAGGCTTCACGTTTGGATCTTGAAAGAACGGCCCGTATCAATTCCTCGGCAGTCTCTGAACAGCTTCCTATAGCAAGGCAAAATTCTGCCTTTGAGAATGGAAGCTTGCCTAGAAAGCTATCAAGCGCAAGCAGCCTTGGAAGCATGGAGGAGAGCTATTTTCTGCAAGCTTCTCTTGATTCTTCAGATAAATTTTCTGAGAAGCGAAGCATGCCTGAGGCAACTATGAGTCCGTACTACATGAAGAGCATCACACCGAGTGCTTATGAAGCCACACTTCGACAGAAAGAGGGTGAACTTGCTTCATACATGACCCGCTTGGCATCAATGGAGTCTATCCGAGATTCCCTGGCTGAAGAACTAGTCAAAATGACAGCAGAGTGTGAGAAGCTACGGGGGGAGGCAGATAGGGTCCCGGGCATAAAGGCAGAGCTGGAAGCACTAAGGCAAAGACACGCGGCTGCTCTGGAATTGATGGGTGAACGAGATGAGGAGTTGGAGGAACTTCGAGCGGATATAGTGGACTTGAAGGAGATGTACAGGGAGCAAGTGAACATGCTGGTGAACAAGATCCAATGA

GATATCGGATCCCGGGCCCGTCGACTGCAGAGGCCTGCATGCAAGCTTGGCGTAATCATGGTCATACCCTATAGTGAGTCGTATTACATGGTCATAGCTG

黑色：载体序列、红色：同源重组序列、绿色：EcoR V酶切位点、蓝色：ORF序列

关键词

Alternative splicing;Coiled coil;Cytoplasm;Golgi apparatus;Phosphoprotein;Reference proteome


质粒图谱

产品说明

本产品是经过离子交换柱纯化后，装在无菌冻存管中通过真空干燥获得的2 μg连有At1g79830(GC5) 基因ORF的质粒DNA干粉。

保存：-20 ℃

使用说明        

1、以5000 × g离心5 min；

2、打开冻存管，加入20 μL无菌水溶解DNA，并在室温下孵育10 min；

3、短暂涡旋冻存管，然后瞬时离心 (小于5000 × g)。

4、质粒溶液在-20℃下可稳定保存一年，请避免反复冻融。


扩增方案

1、收到质粒干粉后请先5000 rpm离心1 min，再加入20 μL无菌水溶解质粒，室温放置1 min；

2、从-80 °C冰箱中取出相应的感受态，置于冰盒上解冻，并做好标记；

3、取2 μL质粒加至100 μL感受态中，冰浴30min;

4、42 °C热激90 s，再冰浴2 min;

5、加入900 μL无抗的LB液体培养基，200 rpm震荡培养45 min;

6、5000 rpm离心5 min，仅留100 μL上清混匀菌体沉淀；

7、混匀后的菌液加至对应抗性的LB平板上，倒入适量玻璃珠，涂匀液体；

8、将平板正向培养1 h，再倒置培养12 h～16 h;

9、挑取单克隆菌落至对应抗性的LB液体培养基中，震荡培养12 h~16 h，根据实验需要提取质粒。

注意事项

1、同一个基因的单个转录序列可能由于发生自然变异 (例如核苷酸多态性)而有所不同，每一种都有其自身有效的存在。

2、该克隆被工程化，但表达取决于基因的性质。

3、本产品仅限于科学研究，不得用于临床诊断或治疗，不得用于食品或药品。

4、为了您的安全和健康，请穿实验服并戴一次性手套操作。

Tair 网站：https://www.arabidopsis.org/servlets/Search?type=generalsearch_action=detailmethod=1show_obsolete=Fname=https://www.arabidopsis.org/servlets/Search?type=general=detail=1=F=At1g79830=gene=4=1sub_type=geneSEARCH_EXACT=4SEARCH_CONTAINS=1
NCBI网站：https://www.ncbi.nlm.nih.gov/gene/?term=https://www.ncbi.nlm.nih.gov/gene/?term=844322