At4g32551 (LUG) ORF Clone

别称：LUG; RON2; At4g32551; L23H3.30

产品类型：ORF克隆载体

基因ID：At4g32551

转录本：AT4G32551.2

载体：pLinker1

抗性：kanR

ORF 长度：2796 bp

蛋白长度：931 aa

蛋白分子量：102233 Da

片段物种：拟南芥（Arabidopsis thaliana）

NCBI gene ID：829390

Uniprot ID：Q9FUY2 

基因摘要

LEUNIG regulates floral organ identity,gynoecium and ovule development. Negatively regulates AGAMOUS . Encodes a glutamine-rich protein with seven WD repeats similar to transcriptional corepressors.

ORF 核苷酸序列

>At4g32551 (LUG) ORF Clone

CTTAAGCTCGGTACCAAGCTTCCTCGAGGGCGCGCCCCGCGGAGATCTTCTAGAGAT

ATGTCTCAGACCAACTGGGAAGCTGATAAAATGTTGGATGTCTATATCCATGATTATCTGGTTAAAAGAGATTTGAAAGCTACAGCTCAGGCTTTCCAAGCTGAAGGAAAAGTGTCATCGGATCCAGTTGCTATTGACGCACCTGGTGGATTTCTGTTTGAGTGGTGGTCTGTCTTCTGGGATATATTTATTGCTAGGACCAATGAGAAGCATTCCGAGGTTGCTGCATCTTACATAGAGACACAGATGATCAAAGCACGAGAACAGCAATTGCAGCAATCTCAACACCCACAAGTTTCACAGCAGCAGCAGCAACAACAACAACAGCAGATTCAAATGCAACAACTCTTGTTGCAACGTGCACAGCAACAGCAGCAGCAGCAACAACAACAACATCATCATCATCAGCAACAGCAACAGCAACAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAACAACAACATCAAAACCAACCACCTTCTCAACAGCAGCAGCAGCAATCAACGCCTCAACACCAACAGCAGCCTACACCACAGCAGCAGCCTCAGAGAAGAGACGGGTCCCACCTTGCAAATGGATCAGCAAATGGACTCGTTGGTAATAACAGTGAACCAGTTATGAGGCAAAATCCTGGGTCTGGGAGTTCCTTAGCTAGTAAAGCATATGAGGAGCGTGTAAAAATGCCTACTCAGAGGGAGTCCTTAGATGAGGCTGCTATGAAGCGATTTGGGGACAATGTTGGACAACTGTTGGACCCAAGTCATGCATCAATATTGAAATCTGCTGCAGCCTCTGGACAGCCTGCAGGGCAAGTGTTACACAGTACAAGTGGTGGTATGTCTCCACAGGTTCAAACTCGAAATCAGCAACTTCCTGGGTCTGCAGTGGATATTAAAAGTGAGATCAATCCCGTGCTCACTCCCAGAACTGCTGTTCCAGAAGGATCGTTGATTGGAATTCCTGGTAGGTTTGCTGTTCTGAGTGTATCATTTCAGTTGGTTAAGCGTGTCAAATGTAGTATAAGTACTTCAGCTCACAAGAATTTGCTTAGGATTAATGACTGGTTTCCTGTTTCAGGTTCAAATCAAGGCAGCAACAACCTTACACTTAAAGGGTGGCCACTCACTGGATTTGATCAGCTTCGTTCTGGTCTTCTTCAGCAGCAAAAACCATTTATGCAGTCTCAGTCTTTTCACCAACTCAACATGCTAACTCCTCAGCATCAGCAACAGCTCATGCTGGCTCAACAGAATCTAAATTCACAGTCCGTCAGTGAAGAGAACAGAAGACTGAAAATGCTATTGAACAACCGGAGCATGACCCTAGGAAAGGATGGCCTTGGGAGTTCTGTAGGGGATGTGTTACCAAATGTCGGATCGTCATTACAACCTGGTGGCTCTCTTTTGCCTCGTGGAGACACCGATATGCTACTTAAGTTAAAGATGGCTCTGTTACAGCAGCAGCAGCAAAATCAACAGCAGGGTGGTGGGAATCCACCACAGCCACAGCCGCAGCCACAACCACTTAATCAGCTTGCCCTTACAAATCCGCAACCACAGAGTTCAAATCACAGTATTCATCAACAAGAGAAATTGGGAGGTGGTGGTAGTATTACGATGGATGGTAGCATTTCAAATTCTTTTCGAGGAAACGAACAGGTATTGAAGAATCAGAGTGGGAGGAAAAGAAAGCAACCGGTTTCGTCTTCTGGACCAGCTAATAGTTCAGGAACAGCTAACACTGCGGGACCATCACCAAGCTCAGCACCTTCTACACCTTCAACTCATACTCCTGGAGATGTGATCTCTATGCCTAATCTGCCGCACAGTGGTGGTTCCTCCAAATCAATGATGATGTTTGGCACTGAAGGAACTGGGACTCTTACATCTCCATCAAATCAGTTGGCTGATATGGATCGATTTGTGGAAGATGGATCACTTGACGACAATGTTGAGTCTTTTTTATCCCAGGAGGATGGTGATCAACGGGACGCTGTTACACGGTGTATGGATGTTAGTAAAGGTTTTACGTTTACTGAAGTGAATTCAGTACGTGCGAGCACAACCAAAGTTACCTGTTGCCACTTCTCATCGGATGGAAAAATGCTTGCTAGTGCTGGACATGACAAAAAGGCTGTACTATGGTACACAGACACTATGAAGCCAAAGACGACCCTAGAGGAGCACACAGCTATGATAACAGATATCCGTTTTAGTCCTAGCCAGCTTCGCCTTGCGACTTCTTCGTTTGACAAGACTGTCAGGGTTTGGGATGCTGATAATAAAGGTTATTCCCTTCGTACTTTCATGGGACATTCTTCTATGGTTACATCACTTGACTTCCATCCCATCAAGGATGATCTTATATGTTCCTGTGACAATGATAACGAAATAAGGTACTGGAGTATCAACAATGGAAGTTGCACAAGAGTGTACAAGGGTGGTTCCACCCAGATAAGGTTCCAACCACGTGTTGGAAAGTATCTTGCGGCTTCATCAGCAAATCTTGTCAATGTACTAGATGTAGAAACACAAGCTATTCGACACTCTTTACAGGGGCATGCTAATCCGATAAACTCGGTTTGCTGGGATCCCTCTGGTGACTTCCTGGCATCTGTTAGTGAAGATATGGTAAAAGTATGGACGCTGGGGACAGGTAGTGAAGGAGAATGTGTTCATGAGCTAAGCTGCAACGGGAACAAATTCCAGTCATGTGTTTTCCATCCGGCTTACCCCTCGCTGCTCGTAATTGGTTGCTACCAGTCTTTAGAACTATGGAACATGTCAGAGAACAAGACGATGACATTACCAGCTCACGAAGGGCTAATTACATCTTTGGCTGTTTCAACTGCAACTGGATTGGTTGCATCAGCTAGTCATGATAAGCTAGTGAAACTGTGGAAGTGA

ATCGAATTCGGGCCCACTAGTCCTGCAGGCGGCCGGGATCCGAGCTC

黑色：载体序列、红色：同源重组序列、蓝色：ORF序列

蛋白简介

Transcription repressor subunit of the SEU-LUG transcriptional corepressor of the C class floral homeotic gene AGAMOUS during the early stages of floral meristem development. Is part of the A class cadastral complex that define the boundaries between the A and C class homeotic genes expression and function. Interacts together with APETALA2 and SEUSS to repress AGAMOUS expression. Also plays a role in ovule and pollen development (PubMed:11782418, PubMed:15277686, PubMed:7743940). Implicated in embryo and floral development (PubMed:18390806). Involved in post-synthesis cell wall modifications necessary for mucilage extrusion from seeds upon imbibition, probably by promoting the expression of genes required for mucilage maturation (PubMed:21362134, PubMed:21402796). Involved in vegetative leaf morphogenesis at later stages of leaf development by restricting cell expansion during leaf growth (PubMed:15208345). Regulates the maintenance on leaf polarity and meristem activity as well as the initiation of embryonic shoot apical meristem (SAM) development (PubMed:19837869).

蛋白质序列

>Transcriptional corepressor LEUNIG (Protein ROTUNDA2)

MSQTNWEADKMLDVYIHDYLVKRDLKATAQAFQAEGKVSSDPVAIDAPGGFLFEWWSVFWDIFIARTNEKHSEVAASYIETQMIKAREQQLQQSQHPQVSQQQQQQQQQQIQMQQLLLQRAQQQQQQQQQQHHHHQQQQQQQQQQQQQQQQQQQQHQNQPPSQQQQQQSTPQHQQQPTPQQQPQRRDGSHLANGSANGLVGNNSEPVMRQNPGSGSSLASKAYEERVKMPTQRESLDEAAMKRFGDNVGQLLDPSHASILKSAAASGQPAGQVLHSTSGGMSPQVQTRNQQLPGSAVDIKSEINPVLTPRTAVPEGSLIGIPGSNQGSNNLTLKGWPLTGFDQLRSGLLQQQKPFMQSQSFHQLNMLTPQHQQQLMLAQQNLNSQSVSEENRRLKMLLNNRSMTLGKDGLGSSVGDVLPNVGSSLQPGGSLLPRGDTDMLLKLKMALLQQQQQNQQQGGGNPPQPQPQPQPLNQLALTNPQPQSSNHSIHQQEKLGGGGSITMDGSISNSFRGNEQVLKNQSGRKRKQPVSSSGPANSSGTANTAGPSPSSAPSTPSTHTPGDVISMPNLPHSGGSSKSMMMFGTEGTGTLTSPSNQLADMDRFVEDGSLDDNVESFLSQEDGDQRDAVTRCMDVSKGFTFTEVNSVRASTTKVTCCHFSSDGKMLASAGHDKKAVLWYTDTMKPKTTLEEHTAMITDIRFSPSQLRLATSSFDKTVRVWDADNKGYSLRTFMGHSSMVTSLDFHPIKDDLICSCDNDNEIRYWSINNGSCTRVYKGGSTQIRFQPRVGKYLAASSANLVNVLDVETQAIRHSLQGHANPINSVCWDPSGDFLASVSEDMVKVWTLGTGSEGECVHELSCNGNKFQSCVFHPAYPSLLVIGCYQSLELWNMSENKTMTLPAHEGLITSLAVSTATGLVASASHDKLVKLWK

关键词

Alternative splicing; Developmental protein; Differentiation; Flowering; Nucleus; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; WD repeat


质粒图谱

产品说明

本产品是经过离子交换柱纯化后，装在无菌冻存管中通过真空干燥获得的2 μg连有At4g32551 (LUG) 基因ORF的质粒DNA干粉。

保存：-20 ℃

使用说明        

1、以5000 × g离心5 min；

2、打开冻存管，加入20 μL无菌水溶解DNA，并在室温下孵育10 min；

3、短暂涡旋冻存管，然后瞬时离心 (小于5000 × g)。

4、质粒溶液在-20℃下可稳定保存一年，请避免反复冻融。


扩增方案

1、收到质粒干粉后请先5000 rpm离心1 min，再加入20 μL无菌水溶解质粒，室温放置1 min；

2、从-80 °C冰箱中取出相应的感受态，置于冰盒上解冻，并做好标记；

3、取2 μL质粒加至100 μL感受态中，冰浴30min;

4、42 °C热激90 s，再冰浴2 min;

5、加入900 μL无抗的LB液体培养基，200 rpm震荡培养45 min;

6、5000 rpm离心5 min，仅留100 μL上清混匀菌体沉淀；

7、混匀后的菌液加至对应抗性的LB平板上，倒入适量玻璃珠，涂匀液体；

8、将平板正向培养1 h，再倒置培养12 h～16 h;

9、挑取单克隆菌落至对应抗性的LB液体培养基中，震荡培养12 h~16 h，根据实验需要提取质粒。

注意事项

1、同一个基因的单个转录序列可能由于发生自然变异 (例如核苷酸多态性)而有所不同，每一种都有其自身有效的存在。

2、该克隆被工程化，但表达取决于基因的性质。

3、本产品仅限于科学研究，不得用于临床诊断或治疗，不得用于食品或药品。

4、为了您的安全和健康，请穿实验服并戴一次性手套操作。