At5g62470(MYB96) ORF Clone

产品内容

pLinker1-At5g62470------------------------2 μg

TE buffer--------------------------------------500 μl

产品信息

产品名称：At5g62470(MYB96) ORF Clone

产品货号：QA5g62470

别称：MYB96;MYBCOV1;At5g62470;K19B1.8

产品类型：ORF克隆载体

基因ID：At5g62470

转录本：AT5G62470.2

载体：pLinker1

抗性：kanR

ORF 长度：1059 bp

蛋白长度：352 aa

蛋白分子量：39188 Da

片段物种：拟南芥（Arabidopsis thaliana）

NCBI gene ID：836367

Uniprot ID：Q24JK1

基因摘要

Encodes a R2R3 type Myb transcription factor whose expression is strongly induced by abscisic acid. Mediates abscisic acid signaling during drought stress response. Promotes seed fatty acid accumulation.

ORF 核苷酸序列

>At5g62470(MYB96) ORF Clone

ACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCCAGTGAATTCGAGCTCGGTACCTCGCGAATGCATCTAGATATC

ATGGGAAGACCACCTTGCTGTGAAAAGATTGGAGTGAAGAAAGGGCCATGGACACCAGAGGAAGACATCATCTTGGTTTCTTACATCCAAGAACATGGTCCTGGAAACTGGAGATCTGTCCCAACACACACAGGTTTGAGAAGATGTAGCAAGAGCTGCAGATTGAGATGGACTAATTATCTTCGACCCGGTATTAAGCGTGGAAATTTTACTGAGCATGAAGAGAAGACAATTGTTCATCTTCAAGCCCTTTTAGGCAACAGATGGGCAGCCATAGCATCATACCTTCCAGAAAGGACAGACAATGATATAAAGAACTATTGGAACACTCACTTGAAGAAGAAGCTCAAAAAGATTAATGAATCTGGTGAAGAAGATAATGATGGTGTCTCTTCATCAAACACTAGTTCACAAAAGAACCATCAAAGCACTAACAAAGGTCAATGGGAAAGAAGACTTCAGACAGACATTAACATGGCAAAACAAGCTCTTTGTGAGGCCTTGTCTTTAGACAAACCATCATCCACTCTTTCATCATCTTCATCATTACCGACACCAGTAATCACACAACAAAACATCCGTAACTTCTCATCAGCTTTGCTTGACCGTTGTTATGATCCATCCTCTTCTTCTTCATCTACCACAACCACCACTACAAGCAACACTACTAATCCATACCCATCAGGGGTATATGCGTCAAGTGCTGAGAACATCGCCCGGTTGCTTCAAGATTTCATGAAAGACACACCCAAGGCTTTAACTTTATCATCTTCATCTCCGGTTTCAGAGACTGGACCACTCACTGCTGCAGTCTCGGAAGAAGGTGGAGAAGGGTTTGAACAATCTTTCTTCAGCTTCAATTCAATGGACGAAACTCAAAACTTGACTCAGGAGACAAGCTTCTTCCATGATCAAGTGATCAAACCGGAAATAACAATGGACCAAGATCATGGTCTAATATCACAAGGGTCTCTGTCTTTGTTTGAGAAATGGTTATTTGATGAGCAAAGCCACGAGATGGTTGGTATGGCACTAGCAGGACAAGAAGGGATGTTCTAG

GATATCGGATCCCGGGCCCGTCGACTGCAGAGGCCTGCATGCAAGCTTGGCGTAATCATGGTCATACCCTATAGTGAGTCGTATTACATGGTCATAGCTG

黑色：载体序列、红色：同源重组序列、绿色：EcoR V酶切位点、蓝色：ORF序列

蛋白简介

Transcription activator involved in the activation of cuticular wax biosynthesis under drought stress. Binds directly to DNA consensus sequences found in the promoters of genes encoding very-long-chain fatty acid-condensing enzymes involved in cuticular wax biosynthesis (PubMed:21398568). Functions together with MYB94 in the activation of cuticular wax biosynthesis (PubMed:27577115). Involved in drought stress response through abscisic acid (ABA) signaling. Mediates ABA signals that enhance plant resistance to drought by reducing stomatal opening. Mediates ABA-auxin cross-talk to regulate lateral root growth under drought stress conditions (PubMed:19625633). Involved in the regulation of ABA biosynthesis and ABA-dependent seed dormancy state. Binds to the promoters of NCED2 and NCED6, which are enzymes catalyzing the first step of ABA biosynthesis (PubMed:25616734). Regulates seed germination by controlling the expression of ABI4, a repressor of lipid breakdown during seed germination (PubMed:25869652). Binds to the promoter of LTP3 and transactivates LTP3 gene in response to drought stress and freezing (PubMed:23404903). Involved in cold stress response. Binds directly to the promoters of heptahelical protein (HHP) genes in response to cold stress. HHPs modulate the expression of SCRM/ICE1, SCRM2/ICE2 and CAMTA3, which are upstream regulators of cold-responsive C-repeat-binding factors (CBFs) (PubMed:25912720). Involved in defense responses against the bacterial pathogen Pseudomonas syringae. May act as a molecular link that mediates cross-talks between ABA and salicylate (PubMed:20149112). Involved in a crosstalk between the circadian clock and ABA signaling. Binds directly to the promoter of APRR1/TOC1 to activate its expression (PubMed:26725725). Promotes ABA signaling (PubMed:30979883). Recruits the histone deacetylase HDA15 to the promoters of a subset of Rho GTPase (ROP) genes, which repress ABA signaling at the early stages of signal transduction (PubMed:30979883). HDA15 represses ROP expression by removing acetyl groups of histone H3 and H4 from the cognate regions, particularly in the presence of ABA (PubMed:30979883). {ECO:0000269|PubMed:19625633, ECO:0000269|PubMed:20149112, ECO:0000269|PubMed:21398568, ECO:0000269|PubMed:23404903, ECO:0000269|PubMed:25616734, ECO:0000269|PubMed:25869652, ECO:0000269|PubMed:25912720, ECO:0000269|PubMed:26725725, ECO:0000269|PubMed:27577115, ECO:0000269|PubMed:30979883}.

蛋白质序列

>Transcription factor MYB96 (Myb-related protein 96) (AtMYB96)

MGRPPCCEKIGVKKGPWTPEEDIILVSYIQEHGPGNWRSVPTHTGLRRCSKSCRLRWTNYLRPGIKRGNFTEHEEKTIVHLQALLGNRWAAIASYLPERTDNDIKNYWNTHLKKKLKKINESGEEDNDGVSSSNTSSQKNHQSTNKGQWERRLQTDINMAKQALCEALSLDKPSSTLSSSSSLPTPVITQQNIRNFSSALLDRCYDPSSSSSSTTTTTTSNTTNPYPSGVYASSAENIARLLQDFMKDTPKALTLSSSSPVSETGPLTAAVSEEGGEGFEQSFFSFNSMDETQNLTQETSFFHDQVIKPEITMDQDHGLISQGSLSLFEKWLFDEQSHEMVGMALAGQEGMF

关键词

Abscisic acid signaling pathway;Activator;Alternative splicing;DNA-binding;Nucleus;Plant defense;Reference proteome;Repeat;Stress response;Transcription;Transcription regulation


质粒图谱

产品说明

本产品是经过离子交换柱纯化后，装在无菌冻存管中通过真空干燥获得的2 μg连有At5g62470(MYB96) 基因ORF的质粒DNA干粉。

保存：-20 ℃

使用说明

1、以5000 × g离心5 min；

2、打开冻存管，加入20 μL无菌水溶解DNA，并在室温下孵育10 min；

3、短暂涡旋冻存管，然后瞬时离心 (小于5000 × g)。

4、质粒溶液在-20℃下可稳定保存一年，请避免反复冻融。


扩增方案

1、收到质粒干粉后请先5000 rpm离心1 min，再加入20 μL无菌水溶解质粒，室温放置1 min；

2、从-80 °C冰箱中取出相应的感受态，置于冰盒上解冻，并做好标记；

3、取2 μL质粒加至100 μL感受态中，冰浴30min;

4、42 °C热激90 s，再冰浴2 min;

5、加入900 μL无抗的LB液体培养基，200 rpm震荡培养45 min;

6、5000 rpm离心5 min，仅留100 μL上清混匀菌体沉淀；

7、混匀后的菌液加至对应抗性的LB平板上，倒入适量玻璃珠，涂匀液体；

8、将平板正向培养1 h，再倒置培养12 h～16 h;

9、挑取单克隆菌落至对应抗性的LB液体培养基中，震荡培养12 h~16 h，根据实验需要提取质粒。

注意事项

1、同一个基因的单个转录序列可能由于发生自然变异 (例如核苷酸多态性)而有所不同，每一种都有其自身有效的存在。

2、该克隆被工程化，但表达取决于基因的性质。

3、本产品仅限于科学研究，不得用于临床诊断或治疗，不得用于食品或药品。

4、为了您的安全和健康，请穿实验服并戴一次性手套操作。

Tair 网站：https://www.arabidopsis.org/servlets/Search?type=general&search_action=detail&method=1&show_obsolete=F&name=At5g62470&sub_type=gene&SEARCH_EXACT=4&SEARCH_CONTAINS=1
NCBI网站：https://www.ncbi.nlm.nih.gov/gene/?term=836367
Uniprot 网站：https://www.uniprot.org/uniprotkb//entry

相关文献 (NCBI PubMed ID)

PMID: 11597504; 9872454; 27862469; 10226370; 19625633; 20149112; 21398568; 23404903; 25912720; 25616734; 25869652; 27577115; 26725725; 30979883