At5g65700(BAM1) ORF Clone

产品内容

pLinker1-At5g65700------------------------2 μg

TE buffer--------------------------------------500 μl

产品信息

产品名称：At5g65700(BAM1) ORF Clone

产品货号：QA5g65700

别称：BAM1;At5g65700;F6H11.170;MPA24.5

产品类型：ORF克隆载体

基因ID：At5g65700

转录本：AT5G65700.1

载体：pLinker1

抗性：kanR

ORF 长度：3012 bp

蛋白长度：1003 aa

蛋白分子量：109203 Da

片段物种：拟南芥（Arabidopsis thaliana）

NCBI gene ID：836699

Uniprot ID：O49545

基因摘要

Encodes a CLAVATA1-related receptor kinase-like protein required for both shoot and flower meristem function. Very similar to BAM2,with more than 85% a.a. identity. It has a broad expression pattern and is involved in vascular strand development in the leaf, control of leaf shape, size and symmetry, male gametophyte development and ovule specification and function. Anthers of double mutants (bam1bam2) appeared abnormal at a very early stage and lack the endothecium, middle, and tapetum layers. Further analyses revealed that cells interior to the epidermis (in anther tissue) acquire some characteristics of pollen mother cells (PMCs), suggesting defects in cell fate specification. The pollen mother-like cells degenerate before the completion of meiosis, suggesting that these cells are defective. In addition, the BAM1 expression pattern supports both an early role in promoting somatic cell fates and a subsequent function in the PMCs. The mRNA is cell-to-cell mobile.

ORF 核苷酸序列

>At5g65700(BAM1) ORF Clone

ACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCCAGTGAATTCGAGCTCGGTACCTCGCGAATGCATCTAGATATC

ATGAAACTTTTTCTTCTCCTTCTTTTTCTTCTCCACATTTCTCATACCTTCACCGCAAGCCGACCAATCTCCGAGTTCCGTGCTCTCCTCTCACTCAAAACCTCTCTCACCGGCGCCGGAGATGACAAAAACTCGCCTCTCTCTTCCTGGAAAGTCTCAACAAGCTTCTGTACATGGATTGGTGTCACGTGCGATGTATCTCGCCGTCACGTGACTTCTCTTGATCTCTCCGGTCTCAACCTCTCCGGTACTCTTTCCCCAGATGTTTCTCATTTACGTCTTCTTCAGAATCTATCACTCGCTGAAAATCTAATCTCCGGTCCGATTCCGCCGGAAATCTCGAGCCTTTCCGGTCTTCGTCACTTAAATCTCTCGAACAATGTCTTCAACGGTTCGTTTCCCGATGAGATTTCTTCTGGATTGGTGAATCTCCGAGTTCTTGATGTCTACAACAACAATCTAACCGGAGATTTACCGGTTTCCGTCACCAATCTGACTCAGCTCCGGCACCTTCACCTCGGTGGTAACTACTTCGCTGGAAAGATCCCGCCTTCGTACGGAAGCTGGCCAGTTATTGAGTATCTAGCGGTTTCCGGCAACGAGCTTGTCGGGAAAATCCCTCCAGAGATCGGAAACCTAACGACTCTCCGGGAGCTTTACATCGGCTACTACAACGCTTTCGAAGACGGTCTTCCTCCAGAGATCGGAAATCTATCGGAGCTAGTCAGATTCGACGGAGCTAATTGCGGATTAACCGGTGAGATTCCGCCGGAGATTGGGAAGCTTCAGAAACTCGATACGCTTTTCTTGCAAGTGAATGTCTTCTCCGGTCCATTAACTTGGGAGCTAGGAACGCTATCGAGTTTAAAATCAATGGATTTGTCTAACAACATGTTCACCGGAGAGATTCCAGCGAGTTTCGCAGAGTTGAAGAATCTCACGCTTTTGAATCTCTTCCGTAACAAACTTCACGGCGAGATACCGGAGTTCATCGGAGATTTGCCGGAGCTTGAAGTGTTACAGCTTTGGGAGAACAATTTCACCGGAAGCATCCCGCAGAAATTAGGAGAAAACGGTAAACTAAATCTCGTCGATCTCTCTTCCAATAAGCTGACCGGAACTTTACCGCCGAACATGTGCTCCGGTAACAAGTTAGAAACTTTAATCACTCTTGGAAACTTTCTCTTTGGTTCAATCCCTGATTCTCTTGGTAAATGTGAGTCTTTGACCCGGATCCGAATGGGTGAGAATTTCCTGAACGGGTCAATCCCAAAAGGACTATTCGGATTACCCAAATTAACTCAAGTGGAGCTCCAAGATAATTATCTCTCCGGAGAGTTACCAGTCGCCGGAGGTGTCTCTGTTAATCTTGGTCAGATCAGTTTATCAAACAACCAGCTCTCAGGTCCATTACCTCCGGCGATCGGGAACTTCACCGGCGTTCAGAAACTTCTTCTTGATGGAAACAAGTTCCAAGGTCCTATTCCTTCAGAGGTAGGGAAGCTTCAGCAGCTCTCGAAGATTGATTTCAGCCACAACTTGTTCTCCGGTCGAATCGCGCCGGAGATTAGTCGTTGCAAGCTCTTAACGTTTGTTGATCTGAGCAGAAACGAGCTCTCCGGTGAAATCCCAAATGAGATCACTGCTATGAAGATATTGAATTACTTGAACCTGTCGAGAAACCATCTGGTTGGATCAATCCCTGGTTCAATCTCGTCAATGCAGAGCTTAACATCTCTTGATTTCTCTTACAACAACCTCTCCGGTTTAGTTCCGGGAACAGGACAATTCAGTTACTTCAACTACACATCGTTCTTGGGTAATCCTGATCTCTGTGGTCCTTATCTTGGTCCTTGTAAAGACGGTGTTGCTAAAGGAGGTCACCAGAGTCATAGTAAAGGACCTTTATCAGCTTCTATGAAGTTATTGCTTGTTCTTGGACTACTTGTTTGTTCGATTGCGTTCGCGGTAGTAGCCATAATCAAAGCTAGATCATTGAAAAAGGCGAGTGAGTCACGGGCTTGGAGGTTAACAGCTTTCCAGAGACTAGACTTCACGTGTGACGATGTCTTGGATTCTCTCAAAGAAGACAACATTATAGGCAAAGGAGGAGCTGGTATTGTCTATAAAGGCGTAATGCCTAATGGTGATCTAGTCGCGGTTAAAAGACTCGCTGCAATGTCTCGTGGATCTTCCCATGATCACGGCTTCAACGCAGAGATTCAAACCTTAGGAAGGATAAGACACAGACACATAGTGAGGCTTCTCGGATTTTGCTCAAACCACGAGACGAATCTACTTGTCTATGAGTACATGCCTAATGGTAGTCTCGGTGAGGTGCTTCACGGTAAGAAAGGAGGACACTTGCATTGGGACACACGTTACAAGATTGCTCTTGAAGCTGCTAAAGGACTCTGTTACCTTCATCACGATTGTTCTCCATTGATCGTTCACAGAGATGTCAAATCAAACAACATCCTCCTTGATTCAAACTTTGAAGCTCATGTTGCTGACTTTGGTCTCGCTAAATTCCTTCAAGATTCCGGTACTTCTGAATGTATGTCTGCAATCGCTGGCTCTTACGGCTACATAGCTCCAGAGTATGCGTATACGTTGAAGGTAGATGAGAAGAGCGATGTGTATAGTTTCGGTGTGGTTCTTTTGGAACTCGTCACCGGAAGAAAACCTGTCGGAGAATTTGGAGACGGTGTCGATATAGTGCAATGGGTTCGTAAAATGACTGATTCGAACAAGGATTCGGTTCTGAAAGTATTGGATCCGAGACTTTCTTCGATTCCGATTCATGAAGTGACGCACGTCTTCTATGTAGCGATGCTCTGTGTAGAAGAACAAGCTGTTGAGAGGCCGACTATGAGAGAAGTTGTTCAGATTCTCACTGAGATCCCGAAGTTGCCACCGTCGAAGGATCAGCCGATGACGGAATCAGCGCCGGAGAGTGAGCTTTCGCCGAAGTCTGGTGTTCAAAGTCCGCCGGATCTACTCAATCTATGA

GATATCGGATCCCGGGCCCGTCGACTGCAGAGGCCTGCATGCAAGCTTGGCGTAATCATGGTCATACCCTATAGTGAGTCGTATTACATGGTCATAGCTG

黑色：载体序列、红色：同源重组序列、绿色：EcoR V酶切位点、蓝色：ORF序列

蛋白简介

Necessary for male gametophyte development, as well as ovule specification and function. Involved in cell-cell communication process required during early anther development, and regulating cell division and differentiation to organize cell layers. Required for the development of high-ordered vascular strands within the leaf and a correlated control of leaf shape, size and symmetry. May regulate the CLV1-dependent CLV3-mediated signaling in meristems maintenance. {ECO:0000269|PubMed:16367950, ECO:0000269|PubMed:16751349, ECO:0000269|PubMed:18780746}.

蛋白质序列

>Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM1 (EC 2.7.11.1) (Protein BARELY ANY MERISTEM 1)

MKLFLLLLFLLHISHTFTASRPISEFRALLSLKTSLTGAGDDKNSPLSSWKVSTSFCTWIGVTCDVSRRHVTSLDLSGLNLSGTLSPDVSHLRLLQNLSLAENLISGPIPPEISSLSGLRHLNLSNNVFNGSFPDEISSGLVNLRVLDVYNNNLTGDLPVSVTNLTQLRHLHLGGNYFAGKIPPSYGSWPVIEYLAVSGNELVGKIPPEIGNLTTLRELYIGYYNAFEDGLPPEIGNLSELVRFDGANCGLTGEIPPEIGKLQKLDTLFLQVNVFSGPLTWELGTLSSLKSMDLSNNMFTGEIPASFAELKNLTLLNLFRNKLHGEIPEFIGDLPELEVLQLWENNFTGSIPQKLGENGKLNLVDLSSNKLTGTLPPNMCSGNKLETLITLGNFLFGSIPDSLGKCESLTRIRMGENFLNGSIPKGLFGLPKLTQVELQDNYLSGELPVAGGVSVNLGQISLSNNQLSGPLPPAIGNFTGVQKLLLDGNKFQGPIPSEVGKLQQLSKIDFSHNLFSGRIAPEISRCKLLTFVDLSRNELSGEIPNEITAMKILNYLNLSRNHLVGSIPGSISSMQSLTSLDFSYNNLSGLVPGTGQFSYFNYTSFLGNPDLCGPYLGPCKDGVAKGGHQSHSKGPLSASMKLLLVLGLLVCSIAFAVVAIIKARSLKKASESRAWRLTAFQRLDFTCDDVLDSLKEDNIIGKGGAGIVYKGVMPNGDLVAVKRLAAMSRGSSHDHGFNAEIQTLGRIRHRHIVRLLGFCSNHETNLLVYEYMPNGSLGEVLHGKKGGHLHWDTRYKIALEAAKGLCYLHHDCSPLIVHRDVKSNNILLDSNFEAHVADFGLAKFLQDSGTSECMSAIAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELVTGRKPVGEFGDGVDIVQWVRKMTDSNKDSVLKVLDPRLSSIPIHEVTHVFYVAMLCVEEQAVERPTMREVVQILTEIPKLPPSKDQPMTESAPESELSPKSGVQSPPDLLNL

关键词

ATP-binding;Cell membrane;Developmental protein;Differentiation;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix


质粒图谱

产品说明

本产品是经过离子交换柱纯化后，装在无菌冻存管中通过真空干燥获得的2 μg连有At5g65700(BAM1) 基因ORF的质粒DNA干粉。

保存：-20 ℃

使用说明

1、以5000 × g离心5 min；

2、打开冻存管，加入20 μL无菌水溶解DNA，并在室温下孵育10 min；

3、短暂涡旋冻存管，然后瞬时离心 (小于5000 × g)。

4、质粒溶液在-20℃下可稳定保存一年，请避免反复冻融。


扩增方案

1、收到质粒干粉后请先5000 rpm离心1 min，再加入20 μL无菌水溶解质粒，室温放置1 min；

2、从-80 °C冰箱中取出相应的感受态，置于冰盒上解冻，并做好标记；

3、取2 μL质粒加至100 μL感受态中，冰浴30min;

4、42 °C热激90 s，再冰浴2 min;

5、加入900 μL无抗的LB液体培养基，200 rpm震荡培养45 min;

6、5000 rpm离心5 min，仅留100 μL上清混匀菌体沉淀；

7、混匀后的菌液加至对应抗性的LB平板上，倒入适量玻璃珠，涂匀液体；

8、将平板正向培养1 h，再倒置培养12 h～16 h;

9、挑取单克隆菌落至对应抗性的LB液体培养基中，震荡培养12 h~16 h，根据实验需要提取质粒。

注意事项

1、同一个基因的单个转录序列可能由于发生自然变异 (例如核苷酸多态性)而有所不同，每一种都有其自身有效的存在。

2、该克隆被工程化，但表达取决于基因的性质。

3、本产品仅限于科学研究，不得用于临床诊断或治疗，不得用于食品或药品。

4、为了您的安全和健康，请穿实验服并戴一次性手套操作。

Tair 网站：https://www.arabidopsis.org/servlets/Search?type=general&search_action=detail&method=1&show_obsolete=F&name=At5g65700&sub_type=gene&SEARCH_EXACT=4&SEARCH_CONTAINS=1
NCBI网站：https://www.ncbi.nlm.nih.gov/gene/?term=836699
Uniprot 网站：https://www.uniprot.org/uniprotkb//entry

相关文献 (NCBI PubMed ID)

PMID: 9628582; 11130714; 27862469; 14593172; 20064227; 14506206; 16751349; 16367950; 18780746; 19376835; 20626648