At4g32700 (TEB) ORF Clone

别称：TEB; At4g32700; F4D11.100

产品类型：ORF克隆载体

基因ID：At4g32700

转录本：AT4G32700.2

载体：pLinker1

抗性：kanR

ORF 长度：6465 bp

蛋白长度：2154 aa

蛋白分子量：238523 Da

片段物种：拟南芥（Arabidopsis thaliana）

NCBI gene ID：829406

Uniprot ID：Q588V7 

基因摘要

Encodes a DNA polymerase required for microhomology mediated repair of DNA double strand breaks and is required for T-DNA integration. The protein contains two conserved functional domains: an N-terminal superfamily II DNA/RNA helicase domain and a C-terminal prokaryotic-type DNA polymerase I domain. Required for regulated cell division and differentiation in meristems. Mutant plants show morphological defects, such as short roots, serrated leaves, and fasciation, as well as defective patterns of cell division and differentiation in the meristem. Mutant plants had 2.5 to 4.5-fold higher expression of ATGR1, ATBRCA1 and RAD51 genes. TEB is required for normal progression of DNA replication and for correct expression of genes during development. Mutant plants are proficient for homologous recombination, homology-directed repair, gene targeting, and transient expression of T-DNA .

ORF 核苷酸序列

>At4g32700 (TEB) ORF Clone

CTTAAGCTCGGTACCAAGCTTCCTCGAGGGCGCGCCCCGCGGAGATCTTCTAGAGAT

ATGGACTCAGATTCTTCTAAATCGCGGATTGATCAGTTTTATGTATCGAAGAAGAGGAAACACCAGTCACCAAATTTAAAGTCTGGGAGGAATGAGAAAAATGTGAAGGTGACAGGGGAAAGATCTCCTGGAGACAAAGGCACTTTGGACAGTTACTTGAAAGCCTCTCTGGATGACAAGAGTACTACCAATAGTGGGTTGCAGGCAAGGCAGGAAGCGTTTACAAGAAAATTGGATTTGGAGGTTTCTGCTTCGTCTGTTGGTCAAAATATACACCCTTGCCTGCCTAAACCTGTAAGTTTTGCAACTTTTAAGGAGTGTTTGGGCCAGAACGGATCTCAAGATTTGCATAAAGAAGGTGTTGCAGCTGAAACTCATGCTACAGATGGTTTACTCTGTGCTAACCAGAAAGATAATTCAGAACTACGAGACTTTGCCACTAGTTTCTTGTCCTTGTATTGTAGTGGAGTGCAGTCTGTTGTTGGCTCACCTCCACATCAGAAGGAAAATGAATTGAAGCGTCGAAGCAGTTCATCATCACTTGCCCAAGATATACAAATTTCTCACAAGAGGCGTTGTGAGTCCGAAAATATACCATCACTAGATGATCTTACAAATCCTCTCGGGAGTAAGCCTGAATCACTCGCTAGGAATGGTAATAATAGAGATAAACCTGTTTCAGACCCTACAAAGAAGATGCCGAGCAATGAATCTGTTGAAATTCCAATGGGCTTGAGGAAGTGTAGTAAGGCGCCAGAATCATCTGCTCATCTAACTGAATTCCACACGCCTGGATCAGCCATTAAATCATGTCCTGTTGGAACCCCTAAGTCAGGATGTGGTAGCTCAATGTTCTCACCTGGTGAGGCTTTCTGGAATGAAGCAATTCAAGTTGCTGATGGTTTGACAATTCCAATTGAAAATTTTGGTTCTGTAGAAGCTAAAGTTAGAGATCAACATGTAACTATTCTTTCATGCAGCAAGAAAACAGATAAATGCACTGAAAAGTTAGAGAGGAGTTTAGATTTAGATGAAATCAGAGTAAAGGATAAAGATGCCATTGGTTTTTCAAAGGTAGTGGAGAAGCATGGGAGAGATTTTAATAAAGAAGTATATCAGCTTCCTGTCAAGAATCTTGAACTTTTGTTTCAAGATAAGAACATTAATGGAGGTATTCAAGAGCGATGTGCTTCATTTGATCAAAATAACATTACTCTAGGAAGCAGTAGGATCTCTGAATCTGCTTTCGTCGGTAATAAAGGATGTGAAAATCTTGATATTGCAAATAATGCTCAGGCAGATAAAGGTTTGATAGGCAAGATGTATCCAGAACCCGAAGGAAAGAAAGTTTTACTGTGTGAGGAAAATCGTGGTGTAAGATCTGTTTCTATGATAAGCAACATGAGGAAACCAGTTGGTTCTAGTGAATCCGAAGAAAGTCATACTCCTTCAAGCTCTCATCGTAATTACGATGGCTTGAGTCTCAGCACTTGGCTTCCCTCAGAAGTTTGCAGTGTATACAATAAGAAAGGAATATCTAAACTCTATCCTTGGCAGGTCGAGTGTCTTCAGGTAGATGGTGTTTTGCAGAAAAGGAATCTAGTATATTGTGCTTCTACAAGTGCTGGTAAAAGTTTTGTTGCAGAAGTCTTGATGCTGCGCCGGGTCATAAGAACTGGAAAAATGGCATTGCTTGTACTACCTTATGTATCCATTTGTGCCGAGAAGGCGGAACACCTTGAGGTCCTTCTTGAACCACTTGGTAAGCATGTTCGTAGTTACTATGGAAATCAGGGTGGTGGAACACTTCCTAAAGACACTTCTGTGGCTGTCTGCACGATTGAGAAGGCAAACTCTTTGATAAATCGCTTGCTTGAAGAGGGTCGTTTGTCAGAACTTGGAATAATAGTTATAGATGAGCTACATATGGTGGGTGACCAACATAGGGGTTATCTTCTGGAACTTATGTTGACGAAACTTCGTTATGCTGCTGGTGAAGGCAGTTCAGAATCAAGTAGTGGTGAGAGTTCAGGGACTAGCAGTGGCAAAGCTGATCCTGCTCATGGCCTACAAATTGTGGGTATGAGTGCTACAATGCCAAATGTTGGCGCAGTTGCTGACTGGCTTCAAGCAGCTTTATATCAGACAGAGTTTCGACCAGTTCCCCTGGAGGAGTATATTAAAGTAGGTAGCACCATTTACAATAAAAAGATGGAAGTTGTGAGGACTATACCAAAAGCTGCTGATATGGGAGGGAAAGACCCGGACCATATAGTTGAGCTTTGTAACGAGGTTGTTCAAGAGGGCAATTCAGTGCTGATATTTTGCTCGAGTCGAAAAGGATGTGAATCAACTGCTAGGCATATTTCAAAGCTCATTAAAAATGTTCCCGTAAACGTTGATGGTGAAAATAGTGAGTTTATGGATATCAGATCTGCTATTGATGCCCTGCGAAGGTCTCCGTCTGGAGTAGATCCTGTACTAGAAGAAACTCTTCCCTCTGGGGTTGCCTATCACCATGCTGGTCTTACAGTAGAAGAAAGAGAGATAGTTGAGACCTGTTACCGTAAGGGTCTTGTACGTGTCTTAACAGCTACATCCACTTTGGCGGCTGGGGTTAACCTGCCTGCAAGGAGGGTTATTTTTCGACAACCAATGATTGGCCGTGATTTTATTGATGGAACAAGGTACAAGCAAATGTCTGGTCGAGCTGGGCGAACTGGGATTGATACAAAAGGAGATAGTGTTTTGATTTGCAAACCTGGAGAACTCAAGCGAATAATGGCACTTCTTAATGAAACCTGCCCACCATTGCAGTCTTGTTTATCTGAGGATAAAAATGGAATGACTCATGCAATACTAGAAGTTGTGGCAGGTGGAATTGTTCAGACTGCCAAAGATATTCATCGCTATGTTAGGTGTACACTTCTGAATTCTACAAAGCCCTTTCAAGATGTGGTCAAATCAGCTCAGGATTCTCTTCGGTGGCTGTGCCACAGGAAATTTCTTGAGTGGAATGAAGAGACAAAGTTATACACTACAACACCCCTTGGACGTGGATCTTTTGGCAGTTCACTCTGCCCAGAGGAGTCACTTATAGTGCTGGATGATCTTCTACGAGCACGTGAAGGACTAGTTATGGCTTCTGATTTGCATCTGGTCTACTTAGTCACACCAATTAATGTTGGTGTTGAGCCAAACTGGGAATTGTATTATGAACGGTTCATGGAACTGTCTCCTTTAGAGCAGTCTGTTGGCAATAGAGTTGGAGTGGTCGAACCTTTCCTGATGCGCATGGCACATGGTGCAACAGTTCGCACTCTAAACAGACCACAGGATGTAAAAAAAAATTTGCGTGGAGAATATGACAGTCGGCATGGTTCGACAAGCATGAAGATGCTTTCAGATGAACAAATGCTTCGAGTGTGCAAAAGATTTTTTGTTGCTCTCATCTTGTCAAAATTAGTTCAGGAAGCTTCTGTGACTGAGGTCTGTGAAGCCTTTAAAGTGGCTAGAGGTATGGTTCAAGCGCTACAGGAGAACGCTGGAAGGTTTTCTTCCATGGTTTCGGTATTCTGTGAGAGGCTTGGATGGCATGATTTGGAAGGATTAGTTGCCAAGTTCCAGAACCGTGTTTCATTTGGTGTCAGGGCTGAGATTGTTGAACTTACTAGCATTCCATATATAAAGGGTTCAAGAGCTAGAGCGCTGTATAAAGCTGGCTTGCGTACATCTCAGGCGATAGCTGAAGCGTCCATCCCTGAAATTGTCAAAGCTCTTTTTGAATCTTCAGCGTGGGCTGCAGAAGGTACTGGACAAAGAAGGATACACTTGGGATTAGCCAAGAAAATCAAGAATGGGGCTCGCAAAATTGTTCTTGAGAAAGCAGAAGAGGCGAGGGCTGCTGCATTTTCTGCTTTTAAGTCACTTGGTTTGGATGTTAATGAGCTTTCAAAGCCCTTGCCGTTGGCTCCTGCTAGTAGTCTCAATGGACAAGAAACTACTGAAAGAGACATTTCCAGAGGCTCTGTTGGTCCCGATGGATTACAACAAAGCATAGAGGGGCACATGGAATGTGAAAACTTTGATATGGATAATCACAGAGAAAAGCCAAGTGAAGTATTAGGTGATGCTACCTTAGGGGTTTCATCAGAAATTAACTTGACCAGTCGTCTTCCTAATTTTAGACCTATAGGAACTGCAGTCGGCACTAATGGGCCTAGTGCTGTTAGTATTCTTTCCAGTGATACTTTCCCTATCCCTGTTTACGATAACAGAGAGATAAAACCTAAGGACAATGTTGAGCAGCATTTGACTAGGAATGACCATATTCCTCTGAGTAGTAATAAGGATGGTACAGGTGAAAAGGGTCCAGTAACTGCAGGAAATATTAGTGGCGGATTTGATTCTTTCTTGGAACTTTGGGGATCTGCTGGCGAATTTTTCTTCGATCTTCACTATAATAAACTACAAGATTTGAATTCACGCATCTCTTATGAGATACATGGAATTGCTATCTGCTGGAATTGCTCCCCTGTATACTATGTCAATCTTAATAAGGATCTTCCCAACCTGGAATGTGTAGAAAAACAAAAGCTTATCGAGGATGCAGTTATTGGCAAGAGTGAGGTTCTGGCTTCTCACAACATGTTGGATGTTATAAAGTCTAGGTGGAACAAAATCAGTAAAATTATGGGGAATGTAAATACGAGAAAGTTCACGTGGAATTTGAAAGTCCAGATTCAGGTGCTTAAGAGTCCCGCCATTTCAATCCAAAGATGCACTCGCTTGAATCTTCCAGAAGGGATCAGGGATGAGTTGGTCGATGGATCTTGGTTAATGATGCCTCCTCTTCACACAAGCCACACCATTGACATGAGCATTGTGATATGGATTCTTTGGCCAGATGAGGAAAGACACTCAAATCCAAACATCGATAAGGAAGTAAAGAAAAGGCTATCCCCAGAGGCAGCTGAAGCTGCTAATCGCAGTGGCAGGTGGAGGAATCAGATCCGAAGGGTTGCCCACAATGGTTGCTGCAGAAGGGTTGCTCAAACGAGAGCCCTTTGTTCAGCACTTTGGAAGATCCTTGTTTCCGAAGAACTTCTTCAAGCACTGACAACCATTGAAATGCCGCTGGTTAATGTCCTTGCAGACATGGAGCTTTGGGGTATAGGTATCGATATAGAAGGATGCCTTAGGGCACGGAATATATTGCGGGATAAACTGCGGTCCCTTGAGAAGAAAGCATTTGAATTGGCTGGCATGACATTTTCATTGCACAACCCAGCTGATATTGCAAATGTGTTGTTTGGACAGTTGAAGTTGCCCATACCTGAAAACCAATCTAAAGGGAAACTTCATCCAAGTACTGATAAGCATTGCTTGGATCTTTTAAGGAATGAGCATCCTGTTGTCCCAATTATCAAAGAACACCGCACTTTGGCAAAGCTCTTAAATTGTACACTTGGATCAATTTGTTCGCTTGCTAAACTACGCTTGAGCACGCAAAGGTACACTTTGCATGGCCGCTGGCTTCAAACATCAACAGCAACTGGGCGGCTTTCCATAGAAGAGCCTAATCTCCAATCTGTTGAGCATGAAGTAGAGTTCAAATTGGATAAAAATGGAAGGGACGTAAGTTCAGATGCTGATCGTTACAAAATAAATGCACGTGATTTCTTTGTTCCTACTCAGGAAAATTGGTTACTCTTGACGGCAGATTATTCACAGATAGAGTTGCGACTTATGGCACACTTTTCTAGAGATTCCTCGTTGATTTCAAAGCTTAGTCAGCCAGAAGGCGATGTCTTTACGATGATAGCTGCAAAATGGACTGGAAAAGCCGAGGATTCTGTTAGTCCACATGATAGAGATCAGACCAAAAGATTGATCTACGGAATTCTTTATGGAATGGGTGCAAACAGACTCGCAGAACAGCTTGAGTGCACCTCAGATGAAGCTAAGGAGAAAATTCGAAGCTTTAAAAGTTCTTTCCCTGCAGTCACCTCTTGGCTAAACGAAACGATTTCATTCTGCCAGGAAAAAGGATACATTCAGACTCTCAAGGGAAGAAGGCGGTTCTTGTCGAAAATAAAATTTGGGAATGCCAAAGAGAAATCGAAGGCTCAAAGGCAAGCTGTAAATTCCATGTGCCAGGGATCTGCTGCTGATATTATCAAGATTGCGATGATAAACATATATTCTGCAATTGCTGAAGATGTTGATACCGCAGCATCTAGTTCTTCAAGTGAAACTAGATTTCACATGTTGAAGGGACGATGCCGAATTCTTCTACAAGTACATGATGAACTTGTGCTGGAAGTTGATCCTTCTTACGTAAAACTAGCTGCAATGTTGCTACAAACCAGCATGGAAAATGCGGTGTCACTTCTTGTCCCTCTTCATGTGAAACTGAAAGTTGGGAAAACATGGGGTTCTTTAGAGCCGTTTCAGACTGATTAA

ATCGAATTCGGGCCCACTAGTCCTGCAGGCGGCCGGGATCCGAGCTC

黑色：载体序列、红色：同源重组序列、蓝色：ORF序列

蛋白简介

Required for regulated cell division and differentiation in meristems and embryos, thus modulating adaxial-abaxial polarity. Regulates the progression of cell cycle and DNA replication (e.g. G2/M progression and intrachromosomal recombination) and the expression of genes during development, especially genes nearby the Helitron transposons.

蛋白质序列

>Helicase and polymerase-containing protein TEBICHI (EC 3.6.4.-)

MDSDSSKSRIDQFYVSKKRKHQSPNLKSGRNEKNVKVTGERSPGDKGTLDSYLKASLDDKSTTNSGLQARQEAFTRKLDLEVSASSVGQNIHPCLPKPVSFATFKECLGQNGSQDLHKEGVAAETHATDGLLCANQKDNSELRDFATSFLSLYCSGVQSVVGSPPHQKENELKRRSSSSSLAQDIQISHKRRCESENIPSLDDLTNPLGSKPESLARNGNNRDKPVSDPTKKMPSNESVEIPMGLRKCSKAPESSAHLTEFHTPGSAIKSCPVGTPKSGCGSSMFSPGEAFWNEAIQVADGLTIPIENFGSVEAKVRDQHVTILSCSKKTDKCTEKLERSLDLDEIRVKDKDAIGFSKVVEKHGRDFNKEVYQLPVKNLELLFQDKNINGGIQERCASFDQNNITLGSSRISESAFVGNKGCENLDIANNAQADKGLIGKMYPEPEGKKVLLCEENRGVRSVSMISNMRKPVGSSESEESHTPSSSHRNYDGLSLSTWLPSEVCSVYNKKGISKLYPWQVECLQVDGVLQKRNLVYCASTSAGKSFVAEVLMLRRVIRTGKMALLVLPYVSICAEKAEHLEVLLEPLGKHVRSYYGNQGGGTLPKDTSVAVCTIEKANSLINRLLEEGRLSELGIIVIDELHMVGDQHRGYLLELMLTKLRYAAGEGSSESSSGESSGTSSGKADPAHGLQIVGMSATMPNVGAVADWLQAALYQTEFRPVPLEEYIKVGSTIYNKKMEVVRTIPKAADMGGKDPDHIVELCNEVVQEGNSVLIFCSSRKGCESTARHISKLIKNVPVNVDGENSEFMDIRSAIDALRRSPSGVDPVLEETLPSGVAYHHAGLTVEEREIVETCYRKGLVRVLTATSTLAAGVNLPARRVIFRQPMIGRDFIDGTRYKQMSGRAGRTGIDTKGDSVLICKPGELKRIMALLNETCPPLQSCLSEDKNGMTHAILEVVAGGIVQTAKDIHRYVRCTLLNSTKPFQDVVKSAQDSLRWLCHRKFLEWNEETKLYTTTPLGRGSFGSSLCPEESLIVLDDLLRAREGLVMASDLHLVYLVTPINVGVEPNWELYYERFMELSPLEQSVGNRVGVVEPFLMRMAHGATVRTLNRPQDVKKNLRGEYDSRHGSTSMKMLSDEQMLRVCKRFFVALILSKLVQEASVTEVCEAFKVARGMVQALQENAGRFSSMVSVFCERLGWHDLEGLVAKFQNRVSFGVRAEIVELTSIPYIKGSRARALYKAGLRTSQAIAEASIPEIVKALFESSAWAAEGTGQRRIHLGLAKKIKNGARKIVLEKAEEARAAAFSAFKSLGLDVNELSKPLPLAPASSLNGQETTERDISRGSVGPDGLQQSIEGHMECENFDMDNHREKPSEVLGDATLGVSSEINLTSRLPNFRPIGTAVGTNGPSAVSILSSDTFPIPVYDNREIKPKDNVEQHLTRNDHIPLSSNKDGTGEKGPVTAGNISGGFDSFLELWGSAGEFFFDLHYNKLQDLNSRISYEIHGIAICWNCSPVYYVNLNKDLPNLECVEKQKLIEDAVIGKSEVLASHNMLDVIKSRWNKISKIMGNVNTRKFTWNLKVQIQVLKSPAISIQRCTRLNLPEGIRDELVDGSWLMMPPLHTSHTIDMSIVIWILWPDEERHSNPNIDKEVKKRLSPEAAEAANRSGRWRNQIRRVAHNGCCRRVAQTRALCSALWKILVSEELLQALTTIEMPLVNVLADMELWGIGIDIEGCLRARNILRDKLRSLEKKAFELAGMTFSLHNPADIANVLFGQLKLPIPENQSKGKLHPSTDKHCLDLLRNEHPVVPIIKEHRTLAKLLNCTLGSICSLAKLRLSTQRYTLHGRWLQTSTATGRLSIEEPNLQSVEHEVEFKLDKNGRDVSSDADRYKINARDFFVPTQENWLLLTADYSQIELRLMAHFSRDSSLISKLSQPEGDVFTMIAAKWTGKAEDSVSPHDRDQTKRLIYGILYGMGANRLAEQLECTSDEAKEKIRSFKSSFPAVTSWLNETISFCQEKGYIQTLKGRRRFLSKIKFGNAKEKSKAQRQAVNSMCQGSAADIIKIAMINIYSAIAEDVDTAASSSSSETRFHMLKGRCRILLQVHDELVLEVDPSYVKLAAMLLQTSMENAVSLLVPLHVKLKVGKTWGSLEPFQTD

关键词

ATP-binding; Cell cycle; Cell division; Developmental protein; Helicase; Hydrolase; Leucine-rich repeat; Nucleotide-binding; Reference proteome; Repeat


质粒图谱

产品说明

本产品是经过离子交换柱纯化后，装在无菌冻存管中通过真空干燥获得的2 μg连有At4g32700 (TEB) 基因ORF的质粒DNA干粉。

保存：-20 ℃

使用说明        

1、以5000 × g离心5 min；

2、打开冻存管，加入20 μL无菌水溶解DNA，并在室温下孵育10 min；

3、短暂涡旋冻存管，然后瞬时离心 (小于5000 × g)。

4、质粒溶液在-20℃下可稳定保存一年，请避免反复冻融。


扩增方案

1、收到质粒干粉后请先5000 rpm离心1 min，再加入20 μL无菌水溶解质粒，室温放置1 min；

2、从-80 °C冰箱中取出相应的感受态，置于冰盒上解冻，并做好标记；

3、取2 μL质粒加至100 μL感受态中，冰浴30min;

4、42 °C热激90 s，再冰浴2 min;

5、加入900 μL无抗的LB液体培养基，200 rpm震荡培养45 min;

6、5000 rpm离心5 min，仅留100 μL上清混匀菌体沉淀；

7、混匀后的菌液加至对应抗性的LB平板上，倒入适量玻璃珠，涂匀液体；

8、将平板正向培养1 h，再倒置培养12 h～16 h;

9、挑取单克隆菌落至对应抗性的LB液体培养基中，震荡培养12 h~16 h，根据实验需要提取质粒。

注意事项

1、同一个基因的单个转录序列可能由于发生自然变异 (例如核苷酸多态性)而有所不同，每一种都有其自身有效的存在。

2、该克隆被工程化，但表达取决于基因的性质。

3、本产品仅限于科学研究，不得用于临床诊断或治疗，不得用于食品或药品。

4、为了您的安全和健康，请穿实验服并戴一次性手套操作。